![]() Shelf Life: 90 days from date of manufacture. Do not use product past the expiration date shown on the label. The expiration date applies to the product in its original packaging and stored as directed. Six genera of Gram-negative rods (Bacteroides, Prevotella, Porphyromonas, Fusobacterium, Bilophila and Sutterella spp.) 2. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. Storage: Upon receipt, store at room temperature in original package until used. T Pigment production may require more than 48 hours incubation. Propionibacterium acnes or Clostridium difficile Enterobacter are gram-negative bacteria that are classified as facultative anaerobes, which means that they are able to thrive in both aerobic and anaerobic. Staphylococcus aureus or Enterococcus faecalis The following organisms are routinely used for quality control performance testing at Anaerobe Systems. Consult reference materials for additional information. A test for aerotolerance should be performed to confirm that each colony type is an obligate anaerobe. A facultative anaerobic organism is an organism that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation if oxygen is absent. Some strains of facultative organisms (which should be inhibited) may grow on LKV. coli cells are typically 1.11.5 m wide, 26 m long and occur as single straight rods. It is recommended that a non-selective medium, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. coli is a member of the family Enterobacteriaceae, which includes Gram-negative facultatively anaerobic rod-shaped bacteria (possessing both a fermentative and respiratory metabolism) and which do not produce the enzyme oxidase. Some organisms that would normally grow on LKV medium may be inhibited. Additional test procedures and media are required for complete identification. LKV agar will not provide complete information for identification of bacterial isolates. LKV agar will support the pigmentation of Prevotella and Porphyromonas species. They are facultative anaerobes and grow optimally at 37☌. Clostridium perfringens and Peptostreptococcus anaerobius). Members of the genus are small, motile, facultatively anaerobic Gram-negative rods. Escherichia coli, Proteus mirabilis, and Staphylococcus aureus) and gram-positive obligate anaerobes (e.g. Prevotella melaningenica and Bacteroides fragilis) and inhibit the growth of facultatively anaerobic bacteria (e.g. If used properly, LKV agar will support good growth of some obligately anaerobic gram-negative bacteria (e.g. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use. Laked sheep blood and vitamin K 1 are added to facilitate the recovery, and pigment production, of Prevotella melaninogenica and Porphyromonas spp. The selective agents include kanamycin and vancomycin to prevent the growth of most obligate gram-negative and gram-positive anaerobic bacteria, along with most facultative anaerobic bacteria. LKV agar contains casein, soy peptone, meat peptone, yeast extract, and dextrose as its nutritive base.
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